Liver Metabolome and Proteome Response of Turbot (Scophthalmus maximus) to Lysine and Leucine in Free and Dipeptide Forms

Omics approaches provide more metabolic information to explain the relationship between dietary nutrition and fish growth. This study aimed explore metabolome proteome response of turbot ( Scophthalmus maximus ) fed diets containing lysine leucine in free dipeptide forms by integrated liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based metabolomics isobaric tags for relative absolute quantification (iTRAQ)-based proteomics. Plant protein-based were formulated contain equivalent amino acid [crystalline (CAA)] synthetic Lys-Leu (Lys-Leu) forms. The profiles liver screened either CAA diet or after an 8-week feeding trial. Fish showed a significantly higher final body weight specific growth rate compared with diet. Protein- acid-related processes identified groups based on differential metabolites proteins. proteolytic enzymes transporters from proteins that process protein digestion absorption may be affected different feed. A mechanistic target rapamycin complex 1 ubiquitin proteasome pathways proteins, which involved synthesis degradation liver. Lysine degradation, tryptophan metabolism, alanine, aspartate, glutamate arginine biosynthesis, proline glycine, serine, threonine metabolism various acids, including lysine, had been both groups. In conclusion, data metabonomics proteomics suggested feed affect absorption, metabolism. addition, good correlation was observed using LC-MS/MS-based iTRAQ-based